Are you sure you want to delete this answer? Yes Sorry, something has gone wrong. Topics for Discussion 1. No I would not expect the Rf values to be different because the pigments will dissolve differently in different types of solvents.
The purpose of this lab experiment was to separate plant pigments using paper chromatography, and to measure the rate of photosynthesis in isolated chloroplasts. Because of capillary action the solvent moves up the paper causing the pigments to become visible at certain distances.
The substances visible on the paper are called pigments. And carotenes and xanthophylls are accessory pigments that make up the rest of the pigmentation.
Carotene is the most soluble of the pigments and as a result will be carried the farthest by the solvent. The paper will display a spectrum of the pigments found in the spinach leaves.
Using the formula Rf one can determine the relationship between the distance the solvent traveled to the distance the pigment traveled. The energy from visible light is used in the photosynthetic process.
The ATP is then used in carbon fixation.
This is the incorporation of CO2 into organic molecules. To measure light transmittance in chloroplasts a spectrophotometer will be used. The reason behind measuring the light transmittance is to calculate the rate of photosynthesis in the chloroplasts. This technique is known as dye- reduction and it tests the hypothesis that light and chloroplasts are required for light reactions to occur.
The materials used in this section of the lab were: The first step was to cut a point on one end of the filter paper and draw a pencil line 1. Next a spinach leave was placed on the strip of paper and rolled over with a quarter on top of the pencil line.
This gives a green line across the paper, which contains the pigments of the leave. Then the strip of paper was placed into the vial with the point down in the bottom.
When the pigment reached the point 1 cm from the top of the vial then it was removed. The solvent front was then quickly marked with a pencil and then each pigment front was marked as well.
From the distance the pigment traveled and the distance the solvent traveled the Rf value was calculated. The materials used in this lab were: First the cuvettes were labeled and cleaned with lens paper because even the oil from your hands can affect the transmittance of light through the cuvette.
Cuvette 2 was then wrapped with foil to keep the contents in the dark.
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Next 1 ml of phosphate buffer was added to all four cuvettes, 4ml of distilled water was added to cuvette 1, 3rn1 of distilled water was added to cuvettes 2,3, and 4, and Iml of DPIP was added to cuvettes 2,3, and 4.
This cuvette was used to recalibrate between readings as well. Three drops of unboiled chloroplasts were placed in cuvette 2 and 3, and three drops of boiled chloroplasts were placed in cuvette 4.Lab 4 Plant Pigments & Photosynthesis.
Introduction: The purpose of this lab experiment was to separate plant pigments using paper chromatography, and to measure the rate of photosynthesis in isolated chloroplasts.
Because of capillary action the solvent moves up the paper causing the pigments to become visible at certain distances.
AP Biology Lab Four: Plant Pigments and Photosynthesis Purpose: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography.
We will also be measuring the rate of photosynthesis in isolated chloroplasts.5/5(43).
AFAM Intro to African American Studies This course provides an overview of African American history and culture. Topics include major events, persons, and issues spanning the period from the African heritage to contemporary times.
Chapter 8 Lab Plant Pigments and Photosynthesis Through the process of photosynthesis, plants convert energy from the sun into energy that is stored in food. Pigments make photosynthesis possible. Chlorophyll is the primary pigment in most plants.
It is within chlorophyll molecules that light energy is . Tip: "I used the new oxygen gas probe for a photosynthesis lab and in less than six minutes using three leaves snipped off a house plant I had the neatest curve you have ever seen.
I have also used the CO2 probe from Vernier with excellent results in photosynthesis and other labs. Respiration lab report - Quick and trustworthy writings from industry leading agency.
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